Perfusion and cryopreservation of whole ovaries with vascular pedicles in common experimental animals

doi:10.16098/j.issn.0529-1356.2019.05.022

Abstract

Abstract:

Objective Using the perfusion needle which is authorized and special perfusion technology to perfuse the whole ovary with vascular pedicle of common animals, such as rabbits, guinea pigs and rats, then comparative analysis of the adjustable perfusion needles whether can be applied to different animals, whether this kind of perfusion pressure and perfusion rate is appropriate for different sizes of ovaries, and using the classic cryopreservation protocol to freeze and thaw. Methods Collecting 12 ovaries respectively from 6 chinese sexual maturity rabbits, 6 Dunkan-Hartley guinea pigs and 6 SD rats to do experiments. Results Through HE staining to count the normal proportion of primordial follicles in each section, the result of HE staining in three animals indicated that there was no statistical significance (P＞0.05), vascular injury was mainly in the upper (far away from ovary), there was no obvious damage in lower (close to ovary) in three animals’ each group. Conclusion The experiment confirms that through adjusting straw needle size we can perfuse different size of animal organs and under the condition of the same perfusion pressure (60 mmHg) and rate (1 ml/min) it is suitable for rabbits’ ovaries, guinea pigs’ ovaries and rats’ ovaries at the same time.

Key words: Whole ovary, Perfusion, Straw needle, HE staining, Rabbit, Guinea pig, Rat

CHEN Wen-ming XU Zhi-hui. Perfusion and cryopreservation of whole ovaries with vascular pedicles in common experimental animals[J]. Acta Anatomica Sinica, 2019, 50(5): 672-676.

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