Effect of Xing-naojing and Shengmai injection on protease activated receptor-1 expression in rats after intracerebral hemorrhage

doi:10.3969/j.issn.0529-1356.2013.05.009

Abstract

Abstract:

Objective To investigate the effect of Xing-naojing and Shengmai injection on protease activated receptor-1(PAR1) expression in rat brain tissue after intracerebral hemorrhage (ICH).Methods The ICH model was induced by injecting blood of its own. Forty male SD rats were randomly divided into ICH group (n=10), saline group (NS, n=10), Xing-naojing and Shengmai injection group (XNJSM, n=10), hirudin group (HIR, n=10). Hematoxylin and eosin staining was performed to examine perihematomal neurocyte morphological changes. The expression of PAR1 in perihematomal tissue was examined by immunohistochemistry and Western blotting techniques. Results The expression of PAR1 in perihematomal tissue was increased after ICH. However,compared with ICH group, perihematomal tissue morphological changes were improved obviously. The expression of PAR1 was decreased significantly between XNJSM group and HIR group(P<0.05). Conclusion XNJSM injection may play a neuroprotective effect by inhibiting the expression of PAR1 protein after ICH.

Key words: Intracerebral hemorrhage, Xing-naojing and Shengmai injection, Protease-activated receptor-1, Immunohistochemistry, Western blotting, Rat

ZHANG Qing JIANG Yan-xia LI Guo-liang WANG Qi-xin MA Cheng-tai* WANG Shou-biao YAO Ru-yong. Effect of Xing-naojing and Shengmai injection on protease activated receptor-1 expression in rats after intracerebral hemorrhage[J]. AAS, 2013, 44(5 ): 630-634.

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