糖尿病下肢血管病变球囊扩张术后再狭窄大鼠模型构建

doi:10.16098/j.issn.0529-1356.2019.01.022

解剖学报 ›› 2019, Vol. 50 ›› Issue (1): 128-131.doi: 10.16098/j.issn.0529-1356.2019.01.022

糖尿病下肢血管病变球囊扩张术后再狭窄大鼠模型构建

邹志伟张倩董建军*

山东大学齐鲁医院内分泌与代谢病科，济南 250012

收稿日期:2018-04-16 修回日期:2018-08-09 出版日期:2019-02-06 发布日期:2019-04-18
通讯作者: 董建军 E-mail:dongjianjun@sdu.edu.cn
基金资助:
Lin28a在糖尿病下肢血管病变球扩后再狭窄中的作用及分子机制研究

Establishment of a diabetic lower extremity arterial disease rat model of restenosis after percutaneous transluminal angioplasty

ZOU Zhi-wei ZHANG Qian DONG Jian-jun*

Department of Endocrinology, Qilu Hospital of Shandong University, Ji’nan 250012, China

Received:2018-04-16 Revised:2018-08-09 Online:2019-02-06 Published:2019-04-18
Contact: DONG Jian-jun E-mail:dongjianjun@sdu.edu.cn

摘要/Abstract

摘要：

目的探讨构建2型糖尿病（T2DM）下肢血管病变球囊扩张术（TPA）后再狭窄的SD大鼠模型的方法。方法 30只T2DM大鼠分为以下3组：动脉粥样硬化组（AS）、再狭窄组（RS）和对照组，HE染色观变血管形态及结构变化。α-平滑肌肌动蛋白（α-SMA）免疫组织化学染色对斑块组成成分进行检测。结果 RS组可见明显的内膜增生，形成再狭窄斑块。构成RS斑块的细胞主要是平滑肌细胞（SMCs）。结论采用球囊拉伤联合球囊扩张术成功构建了糖尿病大鼠下肢血管病变PTA后RS动物模型。

关键词: 糖尿病, 再狭窄, 血管平滑肌, 球囊扩张术, HE染色, 免疫组织化学法, 大鼠

Abstract:

Objective To establish an animal model of restenosis in diabetic lower extremity arterial disease rat after percutaneous transluminal angioplasty(PTA). Methods We divided diabetic rats(n=30) into three groups: atherosclerosis group(AS), restenosis group(RS) and control group. We examined the artery by hematoxylin and eosin(HE), and detected the plaque components by αsmooth muscle actin(α-SMA) immunohistochemical staining. Results Artery restenosis rat models were successfully developed. The smooth muscle cells (SMCs) were the key components of the RS plaque. Conclusion An experimental animal model of restenosis in diabetic lower extremity arterial disease rat after PTA is set up by the steps described in this study.

Key words: Diabetes, Restenosis, Vascular smooth muscle cell, Percutaneous transluminal angioplasty, HE staining, Immunohistochemistry, Rat

邹志伟张倩董建军 . 糖尿病下肢血管病变球囊扩张术后再狭窄大鼠模型构建[J]. 解剖学报, 2019, 50(1): 128-131.

ZOU Zhi-wei ZHANG Qian DONG Jian-jun. Establishment of a diabetic lower extremity arterial disease rat model of restenosis after percutaneous transluminal angioplasty[J]. Acta Anatomica Sinica, 2019, 50(1): 128-131.

参考文献

［1］ Matsuzaki K, Hayashi R, Okabe K, et al. Prognosis of critical limb ischemia: Major vs minor amputation comparison［J］.Wound Repain Regen,2015,23(5):759-764.

［2］ Aiello A, Anichini R, Brocco E, et al. Treatment of peripheral arterial disease in diabetes: a consensus of the Italian Societies of Diabetes (SID, AMD), Radiology (SIRM) and Vascular Endovascular Surgery (SICVE)［J］.Nutr Metab Cardiovasc Dis,2014,24(4):355-369.

［3］ S?derstr?m MI, Arvela EM, Korhonen M, et al. Infrapopliteal Percutaneous transluminal angioplasty versus bypass surgery as firstline strategies in criticallegation: a propensity score analysis ［J］. Ann Surg, 2010,252 (5): 765-773.

［4］ Liistro F, Porto Ⅰ, Angioli P, et al. Drug-eluting balloon in peripheral intervention for below the knee angioplasty evaluation (DEBATE-BTK): a randomized trial in diabetic patients with critical limb ischemia［J］. Circulation, 2013, 128(6):615-621.

［5］ Schillinger M, Exner M, Mlekusch W, et al. Vascular inflammation and percutaneous transluminal angioplasty of the femoropopliteal artery: association with restenosis［J］. Radiology, 2002, 225(1): 21-26.

［6］ Jamsen T, Manninen H, Tulla H, et al. The final outcome of primary infrainguinal percutaneous transluminal angioplasty in 100 consecutive patients with chronic critical limb ischemia［J］. J Vasc Interv Radio, 2002, 13(5): 455-463.

［7］ Fang D, Lin HSh, Yu F, et al. Anatomy of the superficial epigastric artery perforator flap in rats［J］. Acta Anatomica Sinica, 2014,45(4):531-535. (in Chinese)

方冬,林海双,余丰,等.大鼠腹壁浅动脉穿支皮瓣的解剖学［J］.解剖学报,2014,45(4):531-535.

［8］ Peng XW, Huang J, Yan HF, et al. A rat model of vascular restenosis after thoracoabdominal aortic injury: an experimental study［J］. Hunan Journal of Traditional Chinses Medicine, 2017, 33(5):150-152. (in Chinese)

彭熙炜,黄娟,阎卉芳,等.建立大鼠胸腹主动脉损伤后血管再狭窄模型的实验研究［J］.湖南中医杂志,2017,33(5):150-152.

［9］ Zhou X, Dong J, Zhang L, et al. Hyperglycemia has no effect on development of restenosis after percutaneous transluminal angioplasty (PTA) in a diabetic rabbit model［J］. Endocrinol, 2015, 224(2):119-125.

［10］Edlin RS, Tsai S, Yamanouchi D, et al. Characterization of primary and restenotic atherosclerotic plaque from the superficial femoral artery: potential role of Smad3 in regulation of SMC proliferation［J］. J Vasc Surg, 2009, 49(5): 1289-1295.

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糖尿病下肢血管病变球囊扩张术后再狭窄大鼠模型构建

Establishment of a diabetic lower extremity arterial disease rat model of restenosis after percutaneous transluminal angioplasty

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