右美托咪定对丙泊酚致新生乳鼠海马神经元突触及Bcl-2表达的影响

doi:10.16098/j.issn.0529-1356.2019.06.003

解剖学报 ›› 2019, Vol. 50 ›› Issue (6): 718-721.doi: 10.16098/j.issn.0529-1356.2019.06.003

右美托咪定对丙泊酚致新生乳鼠海马神经元突触及Bcl-2表达的影响

严莉汪静雍峻肖红梅曾庆繁*

贵州医科大学附属白云医院麻醉科，贵阳 550058

收稿日期:2018-12-24 修回日期:2019-06-12 出版日期:2019-12-06 发布日期:2019-12-06
通讯作者: 曾庆繁 E-mail:zqfjky@126.com
基金资助:
贵州省科学技术厅项目名称：右美托咪定对丙泊酚致新生大鼠海马神经凋亡的影响

Effects of dexmedetomidine in protective effects of propofol on newborn rats hippocampal synapses and the influence of the Bcl-2 expression

YAN Li WANG Jing YONG Jun XIAO Hong-mei ZENG Qing-fan*

Department of Anesthesiology, Baiyun Hospital Affiliated to Guizhou Medical University, Guiyang 550058, China

Received:2018-12-24 Revised:2019-06-12 Online:2019-12-06 Published:2019-12-06
Contact: ZENG Qing-fan E-mail:zqfjky@126.com

摘要/Abstract

摘要：

目的探讨右美托咪定对丙泊酚致新生乳鼠海马神经元突触及Bcl-2表达的影响。方法分离纯化神经元，并用微管相关蛋白-2（MAP-2）抗体进行鉴定。实验分为正常对照组，丙泊酚组（再加入12 mg/L丙泊酚），丙泊酚+右美托咪定组（加入12 mg/L丙泊酚和0.25 mg/L右美托咪定）。Real-time PCR和Western blotting检测各组细胞Bcl-2的表达；透射电子显微镜检测细胞突触情况。结果成功分离大鼠海马神经元。对照组海马神经细胞以多个突起的锥体细胞为主，胞体呈三角形或椭圆形，体积较大胞体亮、透光性强，周边有光晕，胞质丰富，核大。与对照组相比，丙泊酚组的Bcl-2的表达明显下降；与丙泊酚组相比， Bcl-2的表达明显上升，差异具有统计学意义（P＜0.05）。结论右美托咪定能够通过影响Bcl-2的表达发挥对海马神经元的保护作用。

关键词: 丙泊酚, 右美托咪定, Bcl-2, 海马神经元, 实时定量聚合酶链反应, 大鼠

Abstract:

Objective To evaluate the role of dexmedetomidine in protective effects of propofol on newborn rats hippocampal synapses and the influence of the Bcl-2 expression. Methods Neurons were separated and purificated and identificated by microtubule associated protein-2 (MAP-2) antibody. Rats were divided into normal control group, propofol group(add 12 mg/L propofol), and propofol + dexmedetomidine group(add 12 mg/L propofol and 0.25 mg/L dexmedetomidine). The expression of Bcl-2 test by Real-time PCR and Western blotting; cell synapse was detected by transmission electron microscope. Results Rat hippocampal neurons were separated successfuly. Hippocampal nerve cells in control group were given priority to with multiple processes of pyramidal cell, the cell body was triangular or elliptic, large cell body bright and pervious to light quality, had a halo around, abundant cytoplasm, nuclear. Compared with the control group, in propofol group the Bcl-2 expression decreased obviously. Compared with the propofol group, the expression of Bcl-2 increased obviously (P<0.05). Conclusion Dexmedetomidine can influence the expression of Bcl-2 so as to play a protective role of hippocampal neurons.

Key words: Propofol, Dexmedetomidine, Bcl-2, Hippocampal neuron, Real-time PCR, Rat

严莉汪静雍峻肖红梅曾庆繁. 右美托咪定对丙泊酚致新生乳鼠海马神经元突触及Bcl-2表达的影响[J]. 解剖学报, 2019, 50(6): 718-721.

YAN Li WANG Jing YONG Jun XIAO Hong-mei ZENG Qing-fan. Effects of dexmedetomidine in protective effects of propofol on newborn rats hippocampal synapses and the influence of the Bcl-2 expression[J]. Acta Anatomica Sinica, 2019, 50(6): 718-721.

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右美托咪定对丙泊酚致新生乳鼠海马神经元突触及Bcl-2表达的影响

Effects of dexmedetomidine in protective effects of propofol on newborn rats hippocampal synapses and the influence of the Bcl-2 expression

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