解剖学报

›› 2010, Vol. 41 ›› Issue (2): 237-240.doi: 10.3969/j.issn.0529-1356.2010.02.014

• 论著 • 上一篇    下一篇

贵州布依族人群三个STR基因座遗传多态性

龙友国1*; 余跃生2 ;龙思方1 ;江家志1   

  1. 1．黔南民族医学高等专科学校生物化学教研室;2．预防医学教研室，贵州 都匀 558003
  • 收稿日期:2009-06-09 修回日期:2009-09-14 出版日期:2010-04-06
  • 通讯作者: 龙友国

Genetic polymorphism of 3 STR loci in Bouyei ethnic group in Guizhou

  1. 1.Department of Biochemistry of Qiannan Medical College for Nationalities, Guizhou Duyun 558003, China;2.Department Preventive Medicine of Qiannan Medical College for Nationalites, Guizhou Duyun 558003,China
  • Received:2009-06-09 Revised:2009-09-14 Online:2010-04-06
  • Contact: LONG You-guo

关键词: 短串联重复序列, 遗传多态性, 聚合酶链反应, 贵州布依族

Abstract: Objective To investigate genetic polymorphism of 3 STR loci in Bouyei ethnic group in Guizhou. Methods The DNAs of 101 healthy unrelated Bouyei individuals in Guizhou were extracted using chelex100 method and were multiplex amplificated by PCR technique and the denatured PAGE was used, the PCR product was typied using silver stain method. The data was statistically analysed by modifiedpowerstat software package. Results A total of 21 alleles and 50 genotypes were observed in the 3 loci and the distributions of these genotypes were consistent with the law of EM>Hardy-Weinberg/EM> equilibrium. The heterozygosity(H) of CSF1PO, TPOX and TH01 loci were 0.7287,0.5423 and 0.6904; the polymorphism information content(PIC) were 0.6805,0.4479 and 0.6426; the discrimination power(DP) were 0.8782,0.7361 and 0.8563 and the probabilities of paternity exclusion (PPE)were 0.6148、0.3643 and 0.5737 respectively. Conclution CSF1PO and THOX are highly genetic polymolphism, which is valuable for population genetic research and forensic individual ident

Key words: Short tandem repeat, Genetic polymorphism, PCR, Bouyei ethnic group 

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