解剖学报

›› 2011, Vol. 42 ›› Issue (2): 232-235.doi: 10.3969/j.issn.0529-1356.2011.02.018

• 论著 • 上一篇    下一篇

DNA甲基转移酶1和组蛋白去乙酰化酶1在子宫内膜异位症在位内膜的表达

王水英1;胡燕军2;周彩云2;黄荷凤2*   

  1. 1. 杭州师范大学临床医学院， 杭州310036;2. 浙江大学医学院附属妇产科医院，杭州310006
  • 收稿日期:2010-08-09 修回日期:2010-09-20 出版日期:2011-04-06
  • 通讯作者: 黄荷凤

Expression of DNA methyltransferase　1 and histone deacetylase 1 in endometrium of women with endometriosis

  1. 1. School of Medicine, Hangzhou Normal University, Hangzhou310036, China;2. Women’s Hospital, School of Medicine, Zhejiang University, Hangzhou310006, China
  • Received:2010-08-09 Revised:2010-09-20 Online:2011-04-06
  • Contact: HUANG He-feng

关键词: 子宫内膜异位症, DNA甲基转移酶1, 组蛋白去乙酰化酶1, 免疫印迹法, 免疫组织化学, 人

Abstract: Objective To investigate the alteration in the expression of DNA methyltransferase 1(DNMT1) and histone deacetylase 1(HDAC1) in endometrium from women with endometriosis and to explore the effects of both DNMT1 and HDAC1 on the endometriosis. Methods DNMT1 was localized with immunohistochemistry，DNMT1 and HDAC1 was quantified with Western blotting in the endometrium of 38 premenopausal women (18 with endometriosis and 20 without endometriosis). Results DNMT1 was predominantly located in the nuclei of glandular epithelial cells and stromal cells. The intensities of the DNMT1 immunohistochemical staining were significantly higher in the women endometrium with endometriosis than those without endometriosis(EM>P/EM><0.01）. DNMT1 and HDAC1 protein levels in the endometrium of women with endometriosis were significantly higher than those without endometriosis throughout the menstrual cycle（EM>P/EM><0.05）. There was a positive correlation between endometrial DNMT1 and HDAC1 levels in the endometriosis（EM>P/EM><0.05）.Conclusion High expression of DNMT1 and HDAC1 in the endometrium sug

Key words: Endometriosis, DNA methyltransferase 1, Histone deacetylase 1, Western blotting, Immunohistochemistry, Human

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