解剖学报

解剖学报 ›› 2014, Vol. 45 ›› Issue (1): 128-133.doi: 10.3969/j.issn.0529-1356.2014.01.025

• 人类学 • 上一篇    下一篇

宁夏回、汉族群体LIN28B基因多态性

裴利国1,屈蕾1,贾飞1,张钏1,郭孟境1陆宏1,2  霍正浩1,2*   

  1. 1. 宁夏医科大学生育力保持教育部重点实验室,宁夏生殖与遗传重点实验室，银川 750004;2. 宁夏医科大学基础医学院，银川 750004
  • 收稿日期:2013-07-08 修回日期:2013-09-21 出版日期:2014-02-06 发布日期:2014-02-06
  • 通讯作者: 裴利国 E-mail:medicalningxia@163.com
  • 基金资助:

    人类指长比发育与雄激素受体基因、HOX基因多态性的关联性研究

LIN28B gene polymorphisms in Ningxia Hui and Han ethnic people

PEI Li-guo1 QU Lei1 JIA Fei1 ZHANG Chuan1 GUO Meng-jing1 LU Hong1,2 HUO Zheng-hao1,2*   

  1. 1.Ningxia Medical University, Key Laboratory of Fertility Preservation and Maintenance，Ministry of Education，Key Laboratory of Heredity and Reproduction of Ningxia, Yinchuan 750004, China;2.Department of Basic Medicine of Ningxia Medical University, Yinchuan 750004, China
  • Received:2013-07-08 Revised:2013-09-21 Online:2014-02-06 Published:2014-02-06
  • Contact: HUO Zheng-hao E-mail:medicalningxia@163.com

摘要：

目的 探讨LIN28B基因rs314277和rs314276位点在宁夏地区回、汉族群体中及不同性别间的分布特征。方法 研究对象来自宁夏医科大学2011级健康学生共705例，其中男性回、汉族分别为144例和167例，女性回、汉族分别为188例和206例。SNPs分型均采用基因测序来检测。 结果 宁夏回族群体LIN28B基因两个多态位点基因型频率及等位基因频率分别为：男性rs314277 (AC:4.5%,CC:38.9%; A:2.3%, C:41.1%); rs314276(AA: 2.7%,AC:21.4%,CC:19.3%; A:13.4%,C:30.0%);女性rs314277(AA: 0.6%,AC:7.5%,CC:48.5%; A:4.4%, C:52.3%); rs314276(AA:3.3%,AC:28.9%,CC:24.4%;A:17.8%,C:38.9%);宁夏汉族群体LIN28B基因两个多态位点基因型频率及等位基因频率分别为：男性rs314277(AC:3.2%, CC:41.6%; A:1.6%, C:43.2%); rs314276(AA: 4.6%,AC:17.4%, CC:22.8%; A:13.3%, C:31.5%);女性rs314277(AC:4.6%, CC:50.7%; A:2.3%, C:52.9%); rs314276(AA: 4.8%,AC:18.8%,CC:31.6%; A:14.2%, C:41.0%)。 结论 宁夏人群LIN28B基因rs314277多态性位点等位基因频率在回、汉族之间差异有显著性(P<0.05)；rs314276多态性位点基因型频率在回、汉族之间差异有显著性(P<0.05)；LIN28B基因两个多态性位点在宁夏不同性别之间差异无显著性(P>0.05)；宁夏人群两个多态性位点基因型和等位基因型频率的分布与人类基因组计划扫描所得不同种族人群相比差异有显著性(P<0.05)。

关键词: LIN28B基因, 基因多态性, 基因测序, 回族, 汉族

Abstract:

Objective To study the frequencies of rrs314277 and rs314276 allele and genotype distribution of the LIN28B gene polymorphisms in the Hui and Han people in Ningxia, and to analyze the distributions of LIN28B gene polymorphisms among different places and ethnicity. Methods A total of 705 subjects were from Ningxia Medical University, including 311 cases of boys, of which 144 cases of the Hui and 167 cases of the Han; 394 cases of girls, including the 188 cases of Hui and the 206 cases of Han were analyzed in this study. The two SNPs were examined by the sequencing methods. Results The genotypes frequencies and allele gene frequency of LIN28B gene in Ningxia Hui population were: male：rs314277 (AC:4.5%,CC:38.9%; A:2.3%, C:41.1%); rs314276 (AA:2.7%,AC:21.4%,CC:19.3%; A:13.4%,C:30.0%);female：rs314277(AA: 0.6%, AC:7.5%,CC:48.5%; A:4.4%, C:52.3%); rs314276(AA:3.3%,AC:28.9%, CC：24.4%; A:17.8%,C:38.9%). The genotypes frequencies and allele gene frequency of LIN28B gene in Ningxia Han population were: male：rs314277(AC:3.2%, CC:41.6%; A:1.6%, C:43.2%); rs314276(AA: 4.6%,AC:17.4%, CC:22.8%; A:13.3%, C:31.5%); female：rs314277(AC:4.6%,CC:50.7%; A:2.3%, C:52.9%); rs314276(AA: 4.8%, AC:18.8%, CC:31.6%; A:14.2%, C:41.0%). Conclusion This study showed a significant difference of allele between the Hui and Han ethnic of Ningxia population in rs314277(P<0.05), a significant difference of genotypes between the Hui and Han ethnic of Ningxia population in rs314276(P<0.05). No significant differentce is found between the male and female of Ningxia population in the two SNPs (P>0.05). The differences of distribution traits of genotype and allele frequencies between Ningxia population and other different etbnic population in LIN28B gene indicate that this two SNPs may have genetics characteristic in different ethnics.

Key words: LIN28B, Gene polymorphism, Sequencing, Hui ethnic, Han ethnic